![]() ![]() ![]() To learn more about our new Ion Exchange chromatography resins, download our newly revised handbook here. ![]() Use HiTrap Capto Q ImpRes for anion exchange chromatography, and HiTrap Capto SP ImpRes for cation exchange chromatography. ION EXCHANGE CHROMATOGRAPHY CaptoTM Q XP is a strong anion exchange resin designed for the purification of large biomolecules such as immunoglobulins (e.g. HiScreen Capto Q ImpRes, HiScreen Q HP, 4.7 ml. HiTrap Capto ImpRes can serve as a universal column for routine work in a research lab. Table 3.23 lists suitable empty columns for packing of Capto media from GE. researching the mode of action of antimalarial drug primaquine.analysing the influence of a mitochondrial protein in mammalian cytosolic Fe-S repair.Since the resolution is similar to Sepharose High Performance, Capto ImpRes can be used in these applications as well, and there is no need to have both columns on hand.Ĭapto ImpRes resin has been used in diverse applications, including: Ion exchange chromatography (IEX) separates proteins with differences in surface charge to give high-resolution separation with high sample loading capacity. HiTrap ® Q FF is prepacked with Q Sepharose Fast Flow, and is a strong anion exchanger for small-scale protein purifications as well as screening of binding and elutions conditions. HiTrap Capto ImpRes columns can be used for viscous samples as well as for other high-resolution IEX chromatography procedures with a broad range of samples. Healthcare, Uppsala, Sweden) to a bed height of 10.0 0.1 cm. In this example, the IEX total run time is 30% shorter with HiTrap Capto Q ImpRes than a HiTrap Q HP, with an otherwise similar performing column.įor a larger image, click here. exchange resins: Q Sepharose FF, Q Sepharose HP, Capto Q and DEAE Sephacel (GE. coli lysate, and allows them to be loaded at higher flow rates in a HiTrap Capto ImpRes than a HiTrap HP column. (Capto ImpRes resin has maximum flow rate of 300 cm/h vs 150 cm/h for Sepharose High Performance.) This allows more flexibility - even in the HiTrap format - for viscous samples such as E. HiTrap Capto ImpRes contains Capto ImpRes high-flow agarose resin, which has much better pressure/flow properties than Sepharose High Performance resin. Utilising a robust, modern and rigid resin - such as Capto Q ImpRes - reduces sample run time significantly by allowing high flow rate with low column backpressure, as seen in the example below. coli extract, can increase back pressure on the column and force you to reduce the flow rate, resulting in longer load times. Efficient, industrial-scale purification of AAV of several subclasses by affinity chromatography. Capto AVB is based on a rigid base matrix, delivering excellent pressure-flow properties to AAV production. Small (34 m) bead size delivers high-performance, high-resolution purifications. Affinity chromatography resin designed for the purification of adeno-associated virus (AAV). Packed with Q Sepharose High Performance strong quarternary ammonium anion exchange resin. The hydrophilic nature of the base matrix ensures low levels of non-specific binding leading to low levels of host cell-derived impurities in. High loading capacities give more productive capture from clarified feedstocks. That is the case when choosing a Capto ImpRes resin for use with viscous samples. HiPrep Q HP is a 20 mL strong anion exchange chromatography column for high resolution small-scale protein purification. Q Sepharose XL is a strong anion exchanger designed for use in the capture steps of biomolecules from clarified feed-stocks. Flexible process design due to large operational window of flow rates and bed heights. GE Healthcare Instructions 71-5017-51 AG HiTrap ion exchange columns HiTrap DEAE FF, 1 ml and 5 ml HiTrap CM FF, 1 ml and 5 ml HiTrap Q FF, 1. Efficient industrial-scale purification based on the well-established Capto platform with traditional ligands. While protein purification has become almost routine, new technology and materials always create opportunity to look for improvements. Capto Q XP is designed for capture and intermediate purification of large biomolecules, such as immunoglobulins IgG, IgM, IgA, in downstream processes. Ion exchange chromatography can be used in the first steps of protein purification for both native proteins and untagged recombinant proteins. The less time spent on purification, the more time you will have to focus on studying your protein. Today’s protein researcher is pressed to deliver results faster than ever before. In a recent webinar, we shared some tips for improving protein purity and recovery while performing IEX chromatography here, we focus on your time. Purified AG with very few residual charged PS (GE Healthcare, Chicago, Ill.Have you ever considered how a resin bead in your chromatography column can affect your ion exchange (IEX) chromatography run time? ![]()
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